Journal: Respiratory Research

Article Title: The role and mechanism of thrombospondin-4 in pulmonary arterial hypertension associated with congenital heart disease

doi: 10.1186/s12931-024-02932-w

Figure Lengend Snippet: Circulating level of THBS4 in patients with PAH-CHD. The resultant data are represented as mean ± SD. * P < 0.05

Article Snippet: A human THBS4 ELISA kit (Cusabio, China) was used to measure the circulating level of THBS4 according to the manufacturer’s instructions.

Techniques:

Journal: Respiratory Research

Article Title: The role and mechanism of thrombospondin-4 in pulmonary arterial hypertension associated with congenital heart disease

doi: 10.1186/s12931-024-02932-w

Figure Lengend Snippet: Expression of THBS4 in PAH rats induced by MCT plus aorto-caval shunt. (A) THBS4 expression in GSE149899. qRT-PCR (B) and Western blot (C) showed the time course of THBS4 expression in PAH rats induced by MCT plus aorto-caval shunt. (D) Double immunofluorescence staining of THBS4 with α-SMA or VWF. Scare bars: 50 μm. CON: the control group; SHAM: the sham group; MS: PAH rats induced by MCT plus aorto-caval shunt (MS) from 1 week (1w) to 4 weeks (4w). The resultant data are represented as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

Article Snippet: A human THBS4 ELISA kit (Cusabio, China) was used to measure the circulating level of THBS4 according to the manufacturer’s instructions.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Double Immunofluorescence Staining, Control

Journal: Respiratory Research

Article Title: The role and mechanism of thrombospondin-4 in pulmonary arterial hypertension associated with congenital heart disease

doi: 10.1186/s12931-024-02932-w

Figure Lengend Snippet: qRT-PCR (A) and Western blot (B) showed the transfection efficiency of THBS4 in PASMCs. (C) The influence of THBS4 on the transformation of phenotype of PASMCs. The resultant data are represented as mean ± SD. *: si-THBS4 vs.si-CON; #: pEX3-THBS4 vs. pEX3-CON. * P < 0.05; *** P < 0.001; # P < 0.05; ## P < 0.01; ### P < 0.001

Article Snippet: A human THBS4 ELISA kit (Cusabio, China) was used to measure the circulating level of THBS4 according to the manufacturer’s instructions.

Techniques: Quantitative RT-PCR, Western Blot, Transfection, Transformation Assay

Journal: Respiratory Research

Article Title: The role and mechanism of thrombospondin-4 in pulmonary arterial hypertension associated with congenital heart disease

doi: 10.1186/s12931-024-02932-w

Figure Lengend Snippet: Effect of THBS4 on the proliferation, apoptosis, and migration of PASMCs. (A) Effect of THBS4 on proliferation of PASMCs, as determined by the EdU assay. Scare bars, 100 μm. (B) Effect of THBS4 on apoptosis of PASMCs, as determined by the Annexin-V flow cytometry. (C) Effect of THBS4 on migration of PASMCs, as determined by the Wound healing assay. Scare bars, 200 μm. (D) Effect of THBS4 on migration of PASMCs, as determined by the Transwell assay. Scare bars, 100 μm. The resultant data are represented as mean ± SD. *: si-THBS4 vs.si-CON; #: pEX3-THBS4 vs. pEX3-CON. * P < 0.05; ** P < 0.01; **** P < 0.0001; # P < 0.05; ## P < 0.01

Article Snippet: A human THBS4 ELISA kit (Cusabio, China) was used to measure the circulating level of THBS4 according to the manufacturer’s instructions.

Techniques: Migration, EdU Assay, Flow Cytometry, Wound Healing Assay, Transwell Assay

Journal: Respiratory Research

Article Title: The role and mechanism of thrombospondin-4 in pulmonary arterial hypertension associated with congenital heart disease

doi: 10.1186/s12931-024-02932-w

Figure Lengend Snippet: Phosphorylation level of PI3K/AKT in PASMCs after inhibition and overexpression of THBS4 in PASMCs. The resultant data are represented as mean ± SD. *: si-THBS4 vs. si-CON; #: pEX3-THBS4 vs. pEX3-CON. * P < 0.5; # P < 0.5

Article Snippet: A human THBS4 ELISA kit (Cusabio, China) was used to measure the circulating level of THBS4 according to the manufacturer’s instructions.

Techniques: Phospho-proteomics, Inhibition, Over Expression

Journal: Respiratory Research

Article Title: The role and mechanism of thrombospondin-4 in pulmonary arterial hypertension associated with congenital heart disease

doi: 10.1186/s12931-024-02932-w

Figure Lengend Snippet: Effect of THBS4 suppression on pulmonary vascular remodeling in PAH rats induced by MCT plus aorto-caval shunt. (A) The AAV transfection efficiency was determined by detecting green fluorescence protein which is encoded by AAV in lung tissues. Scare bars, 100 μm. (B) THBS4 suppression efficiency determined by immunofluorescence staining of THBS4. Scare bars, 50 μm. (C) Hematoxylin and Eosin staining of lung tissues. Scare bars,100 μm. (D) Immunofluorescence staining of α-SMA in lung tissues. Scare bars,100 μm. The percentage of non-muscularized (E) , partially-muscularized (F), and fully-muscularized pulmonary arterioles (G) . (H) The percentage of wall thickness. (I) The percentage of wall area. (G) Cross-sectional area of the right ventricular cardiomyocyte. Scare bars,100 μm. The resultant data are represented as mean ± SD. CON: control group; SHAM: sham group; MS-AAV-CON: administration of control AAV + MCT plus aorto-caval shunt induced PAH rats; MS-AAV-siTHBS4: administration of THBS4 suppression AAV + MCT plus aorto-caval shunt induced PAH rats. PA: pulmonary arteriole. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

Article Snippet: A human THBS4 ELISA kit (Cusabio, China) was used to measure the circulating level of THBS4 according to the manufacturer’s instructions.

Techniques: Transfection, Fluorescence, Immunofluorescence, Staining, Control